Research Paper Volume 13, Issue 12 pp 16105—16123

Melatonin protects against focal cerebral ischemia-reperfusion injury in diabetic mice by ameliorating mitochondrial impairments: involvement of the Akt-SIRT3-SOD2 signaling pathway

Effects of melatonin on mitochondrial oxidative stress in diabetic mice with CIR injury. (A) Changes in the production of reactive oxygen species (ROS) were revealed by DHE staining (×400). Scale bar = 50 μm. (B) Quantification of ROS production in the brain. (C–E) Quantitative analysis of levels of mitochondrial malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were quantified by using commercial kits. Data were presented as the mean ± SEM (n = 6). &p #p $p

Figure 2. Effects of melatonin on mitochondrial oxidative stress in diabetic mice with CIR injury. (A) Changes in the production of reactive oxygen species (ROS) were revealed by DHE staining (×400). Scale bar = 50 μm. (B) Quantification of ROS production in the brain. (CE) Quantitative analysis of levels of mitochondrial malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were quantified by using commercial kits. Data were presented as the mean ± SEM (n = 6). &p < 0.05 vs. sham group; #p < 0.05 vs. vehicle group; $p < 0.05 vs. Mel group.