Figure 2. Effects of melatonin on mitochondrial oxidative stress in diabetic mice with CIR injury. (A) Changes in the production of reactive oxygen species (ROS) were revealed by DHE staining (×400). Scale bar = 50 μm. (B) Quantification of ROS production in the brain. (C–E) Quantitative analysis of levels of mitochondrial malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were quantified by using commercial kits. Data were presented as the mean ± SEM (n = 6). &p < 0.05 vs. sham group; #p < 0.05 vs. vehicle group; $p < 0.05 vs. Mel group.