Figure 3. Effects of melatonin on mitochondrial defects in diabetic mice with CIR injury. (A) Representative images of the ultrastructural changes of mitochondria under electron microscopy (×1.2k). (B) Representative images for Cytoplasmic cytochrome C (Cyt-cyto C) expression detected by Western blot. (C) Quantitative analysis of the Cyt-cyto C protein levels. (D) Quantitative analysis of mitochondrial ATP content. (E) Representative images for NRF1 and TFAM expression detected by Western blot. (F, G) Quantitative analysis of the NRF1 and TFAM levels. Data were presented as the mean ± SEM (n = 6). &p < 0.05 vs. sham group; #p < 0.05 vs. vehicle group; $p < 0.05 vs. Mel group.