Research Paper Volume 13, Issue 12 pp 16105—16123

Melatonin protects against focal cerebral ischemia-reperfusion injury in diabetic mice by ameliorating mitochondrial impairments: involvement of the Akt-SIRT3-SOD2 signaling pathway

Effects of Akt signaling on the SIRT3/SOD2 pathway in the neuroprotective effects of melatonin in diabetic mice with CIR injury. (A) Representative images for Akt phosphorylation, SIRT3 expression, and SOD2 acetylation detected by Western blot. (B–D) Quantitative analysis of the ratio of p-Akt/Akt, expression of SIRT3 and the ratio of ac-SOD2/SOD2 in ischemic cortical tissue. Data were presented as the mean ± SEM (n = 6). &p #p $p

Figure 4. Effects of Akt signaling on the SIRT3/SOD2 pathway in the neuroprotective effects of melatonin in diabetic mice with CIR injury. (A) Representative images for Akt phosphorylation, SIRT3 expression, and SOD2 acetylation detected by Western blot. (BD) Quantitative analysis of the ratio of p-Akt/Akt, expression of SIRT3 and the ratio of ac-SOD2/SOD2 in ischemic cortical tissue. Data were presented as the mean ± SEM (n = 6). &p < 0.05 vs. sham group; #p < 0.05 vs. vehicle group; $p < 0.05 vs. Mel group.