Figure 6. Effects of LY294002 and siSIRT3 on the melatonin-mediated reduction in mitochondrial oxidative stress in high glucose-treated HT22 cells with SIR injury. (A) Representative images for ROS generation assessed by flow cytometry. (B) Quantitative analysis of ROS production. (C–E) Quantitative analysis of levels of mitochondrial MDA, SOD and CAT were quantified by using commercial kits. Data were presented as the mean ± SEM (n = 6). ap < 0.05 vs. control group; bp < 0.05 vs. SIR+V group; cp < 0.05 vs. SIR+Mel group.