Figure 3. OGD promotes PTEN nuclear translocation. PTEN nuclear translocation is an essential step in the modulation of ERK1/2 and NF-κB activation. (A) Immunofluorescence staining of GFP in GFP-PTENK13R neurons. The GFP signal was predominantly cytoplasmic, and seldom observed in the nucleus. (B) The whole cell protein levels of PTEN in PTENK13R neurons. Western blot identified a normal sized PTEN protein in whole cell extracts. (C) Qualification of fluorescence intensity of PTEN, normalized against GFP-PTENWT neurons. (D) Qualification of fluorescence intensity of nuclear PTEN versus cytoplasmic PTEN, normalized against GFP-PTENWT neurons. n = 5 in each column and ** p < 0.01, vs. WT. GFP, green fluorescent protein; OGD: oxygen and glucose deprivation, WT: GFP-PTENWT neurons, K13R: GFP-PTENK13R neurons; P: PDTC.