Research Paper Volume 13, Issue 12 pp 16165—16177

PTEN nuclear translocation enhances neuronal injury after hypoxia-ischemia via modulation of the nuclear factor-κB signaling pathway

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Figure 5.

PTEN nuclear translocation is an essential step in NF-κB activation. (A) The expression of NF-κB and p-REK1/2 after PDTC treatment. Western blot of cytoplasmic and nuclear fractions shows that the nuclear translocation of NF-κB decreased in PDTC-treated neurons, while there was no obvious change in the nuclear translocation of PTEN or the expression of p-ERK1/2. (B) Quantification of nuclear and cytoplasmic PTEN expression, normalized against normal neurons. (C) Quantification of nuclear and cytoplasmic p-ERK1/2 expression, normalized against GFP-PTENWT neurons. n = 5 in each column and ** p < 0.01, vs. WT; ##p < 0.01, vs. WT. OGD: oxygen and glucose deprivation, WT: GFP-PTENWT neurons, K13R: GFP-PTENK13R neurons; P: PDTC.