Research Paper Volume 13, Issue 11 pp 14829—14842

Effect of samul-tang on female fertility via RAS signaling pathway in ovaries of aged mice

Quality and quantity of mouse oocytes retrieved after Samul-tang (SM) administration. (A) Eight-week-old mice were orally administered distilled water (n = 6, YC group). Forty-week-old mice were orally administered distilled water (n = 10, OC group) or 2.5 g/kg of SM (n = 10, OC+SM group) five times a week for four weeks. Post SM administration, the mice were superovulated via hormonal stimulation. (B) Oocytes retrieved from the YC, OC, and OC+SM mice at 18 h after hCG injection. Number of retrieved oocytes (C), mature metaphase II (MII) oocytes (D), and MII oocytes with normal chromosomal and spindle alignment (E), retrieved from the three different groups of mice. Data are presented as mean ± standard error of the mean. Statistical analysis was performed using the Student’s t-test.

Figure 3. Quality and quantity of mouse oocytes retrieved after Samul-tang (SM) administration. (A) Eight-week-old mice were orally administered distilled water (n = 6, YC group). Forty-week-old mice were orally administered distilled water (n = 10, OC group) or 2.5 g/kg of SM (n = 10, OC+SM group) five times a week for four weeks. Post SM administration, the mice were superovulated via hormonal stimulation. (B) Oocytes retrieved from the YC, OC, and OC+SM mice at 18 h after hCG injection. Number of retrieved oocytes (C), mature metaphase II (MII) oocytes (D), and MII oocytes with normal chromosomal and spindle alignment (E), retrieved from the three different groups of mice. Data are presented as mean ± standard error of the mean. Statistical analysis was performed using the Student’s t-test.