Figure 4. Hierarchical clustering and analysis of differentially expressed messenger RNAs (mRNAs). (A) QuantSeq 3’ mRNA analysis was performed to compare the gene expression in ovulated ovaries of YC (n = 6), OC (n = 6), and OC+SM (n = 6) mice. (B) Hierarchical clustering among the mRNA expression profiles showing 2,389 differentially expressed mRNAs in the three groups, with a fold-change >1.5 and P< 0.05. (C) Venn diagram presenting the numbers of differentially expressed mRNAs between OC vs. YC and OC+SM vs. OC pairs. YC: 8-week-old control mice; OC: 40-week-old mice; OC+SM: 40-week-old mice orally administered Samul-tang. Up, upregulated genes between compared sets; Contra, contraregulated genes between compared sets; Down, downregulated genes between compared sets.