Validation of expression of differentially expressed mRNAs involved in ovarian function. Quantitative polymerase chain reaction was performed to validate the expression of Fgfr2, Rasgrf1, Egf, Rasal1, Sch3, Nf1, Fgf13, Gng8, and Rasgrf2 in the ovaries of YC, OC and OC+SM mice. (A) Aging-induced downregulated expression of Fgfr2, Rasgrf2 and Egf were restored with SM administration. (B) Aging-induced upregulated expression of Rasal1 and Sch3 were restored with SM administration. (C) Expression of Nf1, Fgf13, Gng8, and Rasgrf1 were changed by SM administration in OC mice. Data are presented as mean ± standard error of the mean. Statistical analysis was performed using the Student’s t-test. YC: 8-week-old control mice; OC: 40-week-old mice; OC+SM: 40-week-old mice orally administered Samul-tang.