Research Paper Volume 13, Issue 12 pp 16229—16247

Testosterone ameliorates age-related brain mitochondrial dysfunction

TP supplementation promotes mitochondrial biogenesis in the SN and HIPP of aged male rats. (A–C) mRNA levels of PGC-1α, NRF-1, and TFAM in the SN. (D) Representative western blots of PGC-1α, NRF-1, and TFAM expression in the SN. (E–G) Quantification of PGC-1α, NRF-1, and TFAM expression in the SN (normalized to β-actin). (H–J) mRNA levels of PGC-1α, NRF-1, and TFAM in the HIPP. (K) Representative western blots of PGC-1α, NRF-1 and TFAM expression in the hippocampus. (L–N) Quantification of PGC-1α, NRF-1, and TFAM expression in the HIPP (normalized to β-actin). Data are expressed as the mean ± S.D. (n = 5/group). *P **P #P U test, Bonferroni correction).

Figure 9. TP supplementation promotes mitochondrial biogenesis in the SN and HIPP of aged male rats. (AC) mRNA levels of PGC-1α, NRF-1, and TFAM in the SN. (D) Representative western blots of PGC-1α, NRF-1, and TFAM expression in the SN. (EG) Quantification of PGC-1α, NRF-1, and TFAM expression in the SN (normalized to β-actin). (HJ) mRNA levels of PGC-1α, NRF-1, and TFAM in the HIPP. (K) Representative western blots of PGC-1α, NRF-1 and TFAM expression in the hippocampus. (LN) Quantification of PGC-1α, NRF-1, and TFAM expression in the HIPP (normalized to β-actin). Data are expressed as the mean ± S.D. (n = 5/group). *P < 0.05, **P < 0.01 (Bonferroni test); #P < 0.0167 (Mann-Whitney U test, Bonferroni correction).