Research Paper Volume 13, Issue 12 pp 16267—16286

Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

LncRNA HCP5 augments cell growth, metastasis, invasion, the epithelial-mesenchymal transition (EMT) process and prohibits hepatocellular carcinoma (HCC) cell apoptosis. (A) Construction of HCP5 knockdown in Hep3B and HCCLM3 cell lines was successful using sh-HCP5 (***pB, C) Compared to the sh-control, knockdown of HCP5 in HCCLM3 and Hep3B cells prohibited cell proliferation by the CCK-8 assay and BrdU assay (*pD, E) Down-regulation of HCP5 decreased migration and invasion in HCCLM3 and Hep3B cell lines (***pF) Cell apoptosis was promoted in HCP5 knockdown cells (***pG) Western blot showing that EMT-related protein E-cadherin was overexpressed in the HCP5 down-regulated group while the expression of vimentin was inhibited.

Figure 2. LncRNA HCP5 augments cell growth, metastasis, invasion, the epithelial-mesenchymal transition (EMT) process and prohibits hepatocellular carcinoma (HCC) cell apoptosis. (A) Construction of HCP5 knockdown in Hep3B and HCCLM3 cell lines was successful using sh-HCP5 (***p<0.001, GAPDH served as the internal control). (B, C) Compared to the sh-control, knockdown of HCP5 in HCCLM3 and Hep3B cells prohibited cell proliferation by the CCK-8 assay and BrdU assay (*p<0.05, **p<0.01, ***p<0.001). (D, E) Down-regulation of HCP5 decreased migration and invasion in HCCLM3 and Hep3B cell lines (***p<0.001, **p<0.01). (F) Cell apoptosis was promoted in HCP5 knockdown cells (***p<0.001). (G) Western blot showing that EMT-related protein E-cadherin was overexpressed in the HCP5 down-regulated group while the expression of vimentin was inhibited.