Research Paper Volume 13, Issue 12 pp 16267—16286

Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

MicroRNA miR-29b-3p is a negatively regulatory target gene of lncRNA HCP5. (A) Bioinformatics predictions demonstrated that a direct HCP5 binding site existed in miR-29b-3p. (B) Dual luciferase assay demonstrating that miR-29b-3p and HCP5 directly interacted (**pC) MiR-29b-3p is expressed in lower-than-usual quantities in hepatocellular carcinoma (HCC) tumor tissues when compared to non-tumor tissues (***pD) Results of qRT-PCR showing that the knockdown of HCP5 increases the expression of miR-29b-3p both in HCCLM3 and Hep3B cell lines (***pE) Results of qRT-PCR showing that up-regulation of miR-29b-3p prohibits the expression of HCP5 (***p

Figure 4. MicroRNA miR-29b-3p is a negatively regulatory target gene of lncRNA HCP5. (A) Bioinformatics predictions demonstrated that a direct HCP5 binding site existed in miR-29b-3p. (B) Dual luciferase assay demonstrating that miR-29b-3p and HCP5 directly interacted (**p<0.01). (C) MiR-29b-3p is expressed in lower-than-usual quantities in hepatocellular carcinoma (HCC) tumor tissues when compared to non-tumor tissues (***p<0.001, U6 served as the internal control). (D) Results of qRT-PCR showing that the knockdown of HCP5 increases the expression of miR-29b-3p both in HCCLM3 and Hep3B cell lines (***p<0.001). (E) Results of qRT-PCR showing that up-regulation of miR-29b-3p prohibits the expression of HCP5 (***p<0.001).