Research Paper Volume 13, Issue 12 pp 16267—16286

Long non-coding RNA HCP5 functions as a sponge of miR-29b-3p and promotes cell growth and metastasis in hepatocellular carcinoma through upregulating DNMT3A

miR-29b-3p precludes hepatocellular carcinoma cell proliferation and migration through enhancing apoptosis and inhibiting the epithelial-mesenchymal transition (EMT) progress. (A) After treatment with miR-29b-3p mimics, HCCLM3 and Hep3B cell lines expressed high quantities of miR-29b-3p when compared to the mimics NC group. (B) Cells with high quantities of miR-29b-3p demonstrating a lower proliferation rate (*pC, D) miR-29b-3p significantly reduces hepatocellular carcinoma (HCC) cell migration and invasion (**pE) Cell apoptosis is improved by miR-29b-3p (***pF) Western blot showing that overexpression of miR-29b-3p results in up-regulation of E-cadherin and reduced level of vimentin.

Figure 5. miR-29b-3p precludes hepatocellular carcinoma cell proliferation and migration through enhancing apoptosis and inhibiting the epithelial-mesenchymal transition (EMT) progress. (A) After treatment with miR-29b-3p mimics, HCCLM3 and Hep3B cell lines expressed high quantities of miR-29b-3p when compared to the mimics NC group. (B) Cells with high quantities of miR-29b-3p demonstrating a lower proliferation rate (*p<0.05, **p<0.01). (C, D) miR-29b-3p significantly reduces hepatocellular carcinoma (HCC) cell migration and invasion (**p<0.01, ***p<0.001). (E) Cell apoptosis is improved by miR-29b-3p (***p<0.001). (F) Western blot showing that overexpression of miR-29b-3p results in up-regulation of E-cadherin and reduced level of vimentin.