Research Paper Volume 13, Issue 14 pp 18191—18222

Formononetin relieves the facilitating effect of lncRNA AFAP1-AS1-miR-195/miR-545 axis on progression and chemo-resistance of triple-negative breast cancer


Figure 4. CDK4 was regulated by lncRNA AFAP1-AS1 and miR-545 in triple-negative breast cancer (TNBC) cells. (A) CDK4 was targeted by miR-545 in certain sites, and luciferase activity of MDA-MB-231 and BT-549 cell lines in the pmirGLO-WT-CDK4+pcDNA6.2/GW/EmGFP-miR-545 group was decreased as relative to pmirGLO-MUT-CDK4+pcDNA6.2/GW/EmGFP-miR-545 group. *: P<0.05. (B, C) Both mRNA and protein levels of CDK4 in MDA-MB-231 and BT-549 cell lines were modulated by pcDNA6.2/GW/EmGFP-miR-545 (B) and pcDNA3.1-lncRNA AFAP1-AS1/si-lncRNA AFAP1-AS1 (C). *: P<0.05.