Figure 6. Raf-1 was implicated in the impact of lncRNA AFAP1-AS1/miR-195 axis on triple-negative breast cancer (TNBC) cells. (A) miR-195 targeted Raf-1 in certain sites, and MDA-MB-231/BT-549 cell lines in the pmirGLO-WT-Raf-1+pcDNA6.2/GW/EmGFP-miR-195 group showed decreased luciferase activity in comparison to pmirGLO-MUT-Raf-1+pcDNA6.2/GW/EmGFP-miR-195 group. *: P<0.05. (B, C) Raf-1 expression in MDA-MB-231 and BT-549 cell lines was affected by pcDNA6.2/GW/EmGFP-miR-195 (B) and pcDNA3.1-lncRNA AFAP1-AS1/si-lncRNA AFAP1-AS1 (C) at mRNA and protein levels. *: P<0.05.