Research Paper Volume 13, Issue 12 pp 16287—16315

Immune infiltration-related N6-methyladenosine RNA methylation regulators influence the malignancy and prognosis of endometrial cancer

Candidate m6A regulator protein and mRNA expression. (A–E) TCGA and UALCAN website analysis of IGF2BP1, ZC3H13, METTL14, ALKBH5, and YTHDC1 mRNA expression in 35 normal and 546 EC tissues, protein expression in 31 normal and 100 EC tissues, and Kaplan-Meier survival curve of the prognostic signature based on five m6A-related genes. *P P P F) Expression of the five genes in 34 EC tissues and 20 normal tissues, as determined by qRT-PCR. (G) Protein expression of ZC3H13, METTL14, and YTHDC1 in 35 EC tissues and 20 normal tissues was determined by immunohistochemistry. DFS curves for ZC3H13, METTL14, and YTHDC1 protein expression in 35 endometrial carcinoma cases. *P P P

Figure 3. Candidate m6A regulator protein and mRNA expression. (AE) TCGA and UALCAN website analysis of IGF2BP1, ZC3H13, METTL14, ALKBH5, and YTHDC1 mRNA expression in 35 normal and 546 EC tissues, protein expression in 31 normal and 100 EC tissues, and Kaplan-Meier survival curve of the prognostic signature based on five m6A-related genes. *P < 0.05, ** P < 0.01, *** P < 0.001. (F) Expression of the five genes in 34 EC tissues and 20 normal tissues, as determined by qRT-PCR. (G) Protein expression of ZC3H13, METTL14, and YTHDC1 in 35 EC tissues and 20 normal tissues was determined by immunohistochemistry. DFS curves for ZC3H13, METTL14, and YTHDC1 protein expression in 35 endometrial carcinoma cases. *P < 0.05, ** P < 0.01, *** P < 0.001.