Research Paper Volume 13, Issue 12 pp 16381—16403

Cardiac senescence is alleviated by the natural flavone acacetin via enhancing mitophagy

Acacetin effects on myocardial telomere length and proteins related to aging and autophagy in senescence mouse model induced by D-galactose. (A) Myocardial telomere length determined by qRT-PCR in mice treated without (control) or with D-galactose (D-gal, 150 mg/kg/day) or D-galactose with acacetin (50 mg/kg/day). (B) Western blots and relative levels of p53 protein in ventricular tissues of mice treated without (control) or with D-galactose or D-galactose with oral 10, 20 or 50 mg/kg acacetin. (C) Western blots and relative levels of p21 protein in ventricular tissues of mice treated as in (B). (D) Western blots and relative levels of PINK1 protein in ventricular tissues of mice treated as in (B). (E) Western blots and relative levels of Parkin protein in ventricular tissues of mice treated as in (B). (F) Western blots and relative levels of Sirt6 protein in ventricular tissues of mice treated as in (B) (n = 6, **P #P ##P

Figure 2. Acacetin effects on myocardial telomere length and proteins related to aging and autophagy in senescence mouse model induced by D-galactose. (A) Myocardial telomere length determined by qRT-PCR in mice treated without (control) or with D-galactose (D-gal, 150 mg/kg/day) or D-galactose with acacetin (50 mg/kg/day). (B) Western blots and relative levels of p53 protein in ventricular tissues of mice treated without (control) or with D-galactose or D-galactose with oral 10, 20 or 50 mg/kg acacetin. (C) Western blots and relative levels of p21 protein in ventricular tissues of mice treated as in (B). (D) Western blots and relative levels of PINK1 protein in ventricular tissues of mice treated as in (B). (E) Western blots and relative levels of Parkin protein in ventricular tissues of mice treated as in (B). (F) Western blots and relative levels of Sirt6 protein in ventricular tissues of mice treated as in (B) (n = 6, **P < 0.01 vs. control. #P < 0.05, ##P < 0.01 vs D-gal).