Research Paper Volume 13, Issue 12 pp 16471—16484

Figure 3. HCG11 acted as a sponge for miR-579-3p in pancreatic carcinoma cells. (A) qPCR was performed to measure miR-579-3p expression in 20 pairs of pancreatic carcinoma tissues and corresponding normal tissues. ^***p<0.001 vs. normal tissues. (B) miR-579-3p expression was also detected in pancreatic carcinoma cell lines (BxPC-3, Capan-2, SW1990, PANC-1 and AsPC-1) by qPCR. ^*p<0.05, ^**p<0.01, ^***p<0.001 vs. HPDE6-C7. (C) Data from qPCR presented that a negative association was observed in HCG11 expression and miR-579-3p expression. ^**p<0.01. (D) The targeting sequences between HCG11 and miR-579-3p were presented. (E, F) The luciferase activity in PANC-1 and AsPC-1 cells was measured by the luciferase reporter assay after transfected with miR-579-3p mimic/NC and HCG11-mut/wt vector. (G, H) qPCR assay was used to detect miR-579-3p expression in pancreatic carcinoma cell lines with up or down regulation of HCG11. ^**p<0.01, ^***p<0.001 vs. siRNA NC, ^##p<0.01 vs. pcDNA3.1.