Research Paper Volume 13, Issue 13 pp 17190—17201

miR-424-5p shuttled by bone marrow stem cells-derived exosomes attenuates osteogenesis via regulating WIF1-mediated Wnt/β-catenin axis

Dampening miR-424-5p expression in exosomes enhanced osteogenic differentiation of BMSCs. MiR-424-5p inhibitors were transfected into BMSCs-derived exosomes, which were then co-cultured with BMSCs for 7 days. (A, B) The expression of miR-424-5p and WIF1 were detected by qRT-PCR. (C) Western blot was adopted to test the protein expression of WIF1/Wnt/β-catenin. (D–F) QRT-PCR was implemented to verify the mRNA expression of OPN, OCN and Runx2. (G) Western blot was employed to monitor the protein expression of OPN, OCN and Runx2. (H) ALP staining. **PPPP

Figure 3. Dampening miR-424-5p expression in exosomes enhanced osteogenic differentiation of BMSCs. MiR-424-5p inhibitors were transfected into BMSCs-derived exosomes, which were then co-cultured with BMSCs for 7 days. (A, B) The expression of miR-424-5p and WIF1 were detected by qRT-PCR. (C) Western blot was adopted to test the protein expression of WIF1/Wnt/β-catenin. (DF) QRT-PCR was implemented to verify the mRNA expression of OPN, OCN and Runx2. (G) Western blot was employed to monitor the protein expression of OPN, OCN and Runx2. (H) ALP staining. **P<0.01, ***P<0.001 (vs. Con(Exo)). &&P<0.01, &&&P<0.001 (vs. Con(Exo)+miR-in).