Priority Research Paper Volume 13, Issue 12 pp 15750—15769

Acid ceramidase promotes senescent cell survival

Stability of the ASAH1 protein in proliferating and senescent cells. (A) Western blot analysis of the levels of ASAH1 and loading control GAPDH in cells harvested after treatment with either vehicle (DMSO) or the protein synthesis inhibitor cycloheximide (CHX) for the times shown. (B) We assessed the levels of ASAH1 and control GAPDH in cells treated with either vehicle (ethanol) or 10 μM MG132 for indicated times by Western blot analysis. To monitor the efficiency of the treatments, we assessed the levels of the labile protein p21.

Figure 4. Stability of the ASAH1 protein in proliferating and senescent cells. (A) Western blot analysis of the levels of ASAH1 and loading control GAPDH in cells harvested after treatment with either vehicle (DMSO) or the protein synthesis inhibitor cycloheximide (CHX) for the times shown. (B) We assessed the levels of ASAH1 and control GAPDH in cells treated with either vehicle (ethanol) or 10 μM MG132 for indicated times by Western blot analysis. To monitor the efficiency of the treatments, we assessed the levels of the labile protein p21.