Figure 9. The expression of the 12 prognostic genes in clinical breast cancer samples. Clinical samples of breast carcinoma tissues and normal breast tissues were collected and followed by RNA extraction for qRT-PCR measurement of relative gene expression of ANLN, CCNE2, KIF4A, MELK, NDC80, NEK2, TOP2A, TTK, UBE2T FREM1, IGF1 and SORBS1. Data are means ± SEM. ns, denotes not significant. *P < 0.05. **P < 0.001.