Research Paper Volume 13, Issue 13 pp 17901—17913

Chloroquine suppresses proliferation and invasion and induces apoptosis of osteosarcoma cells associated with inhibition of phosphorylation of STAT3

CQ induces the apoptosis and inhibits the viability and proliferation of OS cells. (A) The OS cell viability at 24 h, 48 h, and 72 h after treated with CQ (n = 3). (B) The IC50 value at 24 h and 48 h in 143B and U-2OS cells (n = 3). (C) The colony formation assay showed that the proliferation of OS cells was inhibited gradually by CQ with the increasing of concentration (***p n = 3). (D) Flow cytometry showed that CQ could regulate apoptosis of OS cells (***p n = 3). All the data were expressed as the mean ± SD.

Figure 1. CQ induces the apoptosis and inhibits the viability and proliferation of OS cells. (A) The OS cell viability at 24 h, 48 h, and 72 h after treated with CQ (n = 3). (B) The IC50 value at 24 h and 48 h in 143B and U-2OS cells (n = 3). (C) The colony formation assay showed that the proliferation of OS cells was inhibited gradually by CQ with the increasing of concentration (***p < 0.001; n = 3). (D) Flow cytometry showed that CQ could regulate apoptosis of OS cells (***p < 0.001; n = 3). All the data were expressed as the mean ± SD.