Research Paper Volume 13, Issue 13 pp 17901—17913

Chloroquine suppresses proliferation and invasion and induces apoptosis of osteosarcoma cells associated with inhibition of phosphorylation of STAT3

CQ suppression of mouse OS cell xenograft growth and lung metastasis in vivo. (A) Xenograft photographs, growth curves of tumor, and tumor weight showed that CQ suppressed xenograft growth in nude mice (*p n = 3). (B) HE staining of mouse lung tissues. The results showed suppression of lung metastasis in vivo (**p n = 3). (C) Representative pictures from IHC assay. p-STAT3, STAT3, Bcl-2, Bax, Ki67, Vimentin, E-cadherin, N-cadherin, Slug, and CD31 expression in the tumors collected from different groups were determined using IHC (ns p > 0.05; *p **p ***p n = 3). The data were expressed as the mean ± SD.

Figure 3. CQ suppression of mouse OS cell xenograft growth and lung metastasis in vivo. (A) Xenograft photographs, growth curves of tumor, and tumor weight showed that CQ suppressed xenograft growth in nude mice (*p < 0.05; n = 3). (B) HE staining of mouse lung tissues. The results showed suppression of lung metastasis in vivo (**p < 0.01; n = 3). (C) Representative pictures from IHC assay. p-STAT3, STAT3, Bcl-2, Bax, Ki67, Vimentin, E-cadherin, N-cadherin, Slug, and CD31 expression in the tumors collected from different groups were determined using IHC (ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; n = 3). The data were expressed as the mean ± SD.