Research Paper Volume 13, Issue 13 pp 17901—17913

Chloroquine suppresses proliferation and invasion and induces apoptosis of osteosarcoma cells associated with inhibition of phosphorylation of STAT3

CQ reduced the protein degradation of Bax and Caspase3, and CQ-induced apoptosis of OS cells was dependent on the lysosomal inhibition. The (A) Representative pictures from tumor cell western blot assay. (B) The graph is the quantified data of the western blot for the expression of BAX, and GAPDH was used as a load control (*p **p n = 3). (C) The graph is the quantified data of the western blot for the expression of Caspase3, and GAPDH was used as a load control (**p ***p n = 3). (D) Representative pictures from 143B cells western blot assay and the cells were treated with PBS, 20 μM CQ, EBSS, or the combination of CQ and EBSS. The data were expressed as the mean ± SD.

Figure 6. CQ reduced the protein degradation of Bax and Caspase3, and CQ-induced apoptosis of OS cells was dependent on the lysosomal inhibition. The (A) Representative pictures from tumor cell western blot assay. (B) The graph is the quantified data of the western blot for the expression of BAX, and GAPDH was used as a load control (*p < 0.05; **p < 0.01; n = 3). (C) The graph is the quantified data of the western blot for the expression of Caspase3, and GAPDH was used as a load control (**p < 0.01; ***p < 0.001; n = 3). (D) Representative pictures from 143B cells western blot assay and the cells were treated with PBS, 20 μM CQ, EBSS, or the combination of CQ and EBSS. The data were expressed as the mean ± SD.