Figure 2. The down-regulation of APBB2 attenuates proliferation, invasion, and cell cycle progression in GA cell lines. (A and B) evaluation of APBB2 expression by PCR and Western blot in GA cell lines following treatment with APBB2-sh-RNA 1,2,3. (C) Cell viability was evaluated using an MTT assay. (D) GA cell proliferation was determined by a colony formation assay. (E) A transwell assay was used to determine the invasion ability of GA cells (4×). (F) FCM was used to analyze the proportion of GA cells in each phase of the cell cycle (APBB2-silenced cells and NC cells). *P < 0.05.