Research Paper Volume 13, Issue 12 pp 16763—16772

Hsa-miR-30a-3p attenuates gastric adenocarcinoma proliferation and metastasis via APBB2

The overexpression of miR-30a-3p inhibits GA development, which can be reversed by APBB2. (A) Expression of miR-30a-3p and APBB2 in transfected cells was detected by qRT-PCR. (B) An MTT assay was used to determine cell viability at 24 h, 48 h, and 72 h, respectively. (C) The proliferation of GA cells was determined by a colony formation assay. (D and E) A transwell assay was used to determine the migration and invasion ability of GA cells(4×); (F) The proportion of GA cells in each stage of the cell cycle was analyzed by FCM. *P **P

Figure 4. The overexpression of miR-30a-3p inhibits GA development, which can be reversed by APBB2. (A) Expression of miR-30a-3p and APBB2 in transfected cells was detected by qRT-PCR. (B) An MTT assay was used to determine cell viability at 24 h, 48 h, and 72 h, respectively. (C) The proliferation of GA cells was determined by a colony formation assay. (D and E) A transwell assay was used to determine the migration and invasion ability of GA cells(4×); (F) The proportion of GA cells in each stage of the cell cycle was analyzed by FCM. *P < 0.05; **P < 0.01.