Research Paper Volume 13, Issue 12 pp 15875—15897

miRNAs generated from Meg3-Mirg locus are downregulated during aging

Age-dependent alteration of representative miRNAs from Meg3-Mirg cluster in several mouse organs (A–F) The expression of miRNAs in cardiac ventricle (A), skeletal muscle (B), liver (C), pancreas (D), kidney (E) and brain (F) in young and very old mice. For each miRNA, the expression is presented relative to the young group; Two-tailed two-samples T-test for equal or unequal variances; Wilcoxon two-sample test for non-normal distribution; * p G) Heat map showing the expression of representative miRNAs normalized to U6 snRNA in mouse organs harvested from young and very old mice; Note that different organs showed variable expression of representative miRNAs (brain > skeletal muscle > pancreas > ventricles > kidney > liver).

Figure 4. Age-dependent alteration of representative miRNAs from Meg3-Mirg cluster in several mouse organs (AF) The expression of miRNAs in cardiac ventricle (A), skeletal muscle (B), liver (C), pancreas (D), kidney (E) and brain (F) in young and very old mice. For each miRNA, the expression is presented relative to the young group; Two-tailed two-samples T-test for equal or unequal variances; Wilcoxon two-sample test for non-normal distribution; * p < 0.05, ** p < 0.01, *** p < 0.001; n=10/group. (G) Heat map showing the expression of representative miRNAs normalized to U6 snRNA in mouse organs harvested from young and very old mice; Note that different organs showed variable expression of representative miRNAs (brain > skeletal muscle > pancreas > ventricles > kidney > liver).