Figure 5. Effect of wt ZNRD1-AS1 or mut ZNRD1-AS1 overexpression on MKN28 cells. Wild-type and mutant ZNRD1-AS1 constructs or an empty vector were transfected into MKN28 cells. ZNRD1-AS1 mRNA expression was measured by qRT-PCR (A). Cell proliferation (B) and apoptosis (C) were analyzed using the CCK8 assay and flow cytometry, respectively. Cell invasion (D) and migration (E) were analyzed using the transwell assay. Left panels show representative micrographs. Right panels show statistical results. *p <0.05, for empty vector vs. wt ZNRD1-AS1; #p <0.05, for mut ZNRD1-AS1 vs. wt ZNRD1-AS1; ns, p >0.05.