Figure 2. Inhibition of ANO5 decreased osteosarcoma cell proliferation and metastasis in vitro. (A, B) qRT-PCR and western blot were used to determine the transfection efficiency of targeted ANO5 shRNA. (C) A CCK-8 assay was used to evaluate the effects of ANO5 knockdown on osteosarcoma cell proliferation. (D–F) EDU assays showed that proliferation rates were reduced in ANO5 knockdown osteosarcoma cells. (G) A wound healing assay was used to examine the effect of ANO5 inhibition on osteosarcoma cell migration. (H) A transwell assay was used to determine the effect of ANO5 knockdown on osteosarcoma cell invasion. *, P<0.05; **, P<0.01.