Research Paper Volume 13, Issue 13 pp 17316—17327

Anoctamin 5 promotes osteosarcoma development by increasing degradation of Nel-like proteins 1 and 2

Overexpression of ANO5 increased osteosarcoma cell proliferation and metastasis in vitro. (A, B) qRT-PCR and western blot were used to evaluate the transfection efficiency of ANO5 overexpression lentivirus. (C) A CCK-8 assay was used to examine the effects of ANO5 overexpression on osteosarcoma cell proliferation. (D–F) EDU assays showed that proliferation rates were higher in ANO5 overexpression osteosarcoma cells. (G) A wound healing assay was used to determine the effect of ANO5 overexpression on osteosarcoma cell migration. (H) A transwell assay was used to determine the effect of ANO5 overexpression on osteosarcoma cell invasion. *, PP

Figure 3. Overexpression of ANO5 increased osteosarcoma cell proliferation and metastasis in vitro. (A, B) qRT-PCR and western blot were used to evaluate the transfection efficiency of ANO5 overexpression lentivirus. (C) A CCK-8 assay was used to examine the effects of ANO5 overexpression on osteosarcoma cell proliferation. (DF) EDU assays showed that proliferation rates were higher in ANO5 overexpression osteosarcoma cells. (G) A wound healing assay was used to determine the effect of ANO5 overexpression on osteosarcoma cell migration. (H) A transwell assay was used to determine the effect of ANO5 overexpression on osteosarcoma cell invasion. *, P<0.05; **, P<0.01.