Research Paper Volume 13, Issue 13 pp 17316—17327

Anoctamin 5 promotes osteosarcoma development by increasing degradation of Nel-like proteins 1 and 2

NELL1 and NELL2 knockdown suppressed ANO5-induced inhibition of proliferation and invasion. (A) Western blot was used to detect the effects of NELL1 and NELL2 inhibition on expression of ANO5, NELL1, and NELL2 in ANO5 knockdown cells. (B) CCK-8 was used to detect the effects of NELL1 and NELL2 knockdown on ANO5 suppression-induced inhibition of cell proliferation. (C) A transwell assay was used to detect the effects of NELL1 and NELL2 knockdown on ANO5 suppression-induced inhibition of cell invasion. *, PP

Figure 6. NELL1 and NELL2 knockdown suppressed ANO5-induced inhibition of proliferation and invasion. (A) Western blot was used to detect the effects of NELL1 and NELL2 inhibition on expression of ANO5, NELL1, and NELL2 in ANO5 knockdown cells. (B) CCK-8 was used to detect the effects of NELL1 and NELL2 knockdown on ANO5 suppression-induced inhibition of cell proliferation. (C) A transwell assay was used to detect the effects of NELL1 and NELL2 knockdown on ANO5 suppression-induced inhibition of cell invasion. *, P<0.05; **, P<0.01.