Figure 6. ZEB1 and ZEB2 collaboratively abrogated the inhibitory effect of miR-144-3p on CRA cell proliferation and invasion. (A) qRT-PCR analysis of ZEB1, ZEB2 expression in LovomiR-144-3p mimic cells with overexpression of ZEB1 or/and ZEB2 and in HCT116miR-144-3p inhibitor cells with knockdown of ZEB1 or/and ZEB2. (B) Western blot analysis of protein expression of ZEB1 and ZEB2 in indicated cells. (C) MTT assay of proliferation ability of LovomiR-144-3p mimic cells. (D) MTT assay of proliferation ability of HCT116miR-144-3p inhibitor cells. (E) Transwell migration and invasion assays of the migration and invasion of LovomiR-144-3p mimic cells. (F) Transwell migration and invasion assay of HCT116miR-144-3p inhibitor cells after knockdown of ZEB1 or/and ZEB2. *, P<0.05; **, P<0.01; ***, P < 0.001.