Research Paper Volume 13, Issue 13 pp 17349—17369

miR-144-3p inhibited the growth, metastasis and epithelial-mesenchymal transition of colorectal adenocarcinoma by targeting ZEB1/2

ZEB1 and ZEB2 collaboratively mediated the effect of miR-144-3p on CRA cell EMT. (A) qRT-PCR analysis of E-cadherin and vimentin expression in LovomiR-144-3p mimic cells with overexpression of ZEB1 or/and ZEB2, and in HCT116miR-144-3p inhibitor cells with knockdown of ZEB1 or/and ZEB2. (B) Protein expression of E-cadherin and vimentin in indicated CRA cells was tested by Western blot analysis. Immunofluorescence (C) and flow cytometry (D) analysis of E-cadherin and vimentin expression in LovomiR-144-3p mimic cells with overexpression of ZEB1 or/and ZEB2, and in HCT116miR-144-3p inhibitor cells with knockdown of ZEB1 or/and ZEB2. (E) Representative IHC images of serial sections showed the expression of E-cadherin, vimentin, ZEB1, ZEB2 in NCMTs and CRATs with high miR-144-3p expression or low miR-144-3p expression determined by the cut-off value of qRT-PCR. *, PPP

Figure 7. ZEB1 and ZEB2 collaboratively mediated the effect of miR-144-3p on CRA cell EMT. (A) qRT-PCR analysis of E-cadherin and vimentin expression in LovomiR-144-3p mimic cells with overexpression of ZEB1 or/and ZEB2, and in HCT116miR-144-3p inhibitor cells with knockdown of ZEB1 or/and ZEB2. (B) Protein expression of E-cadherin and vimentin in indicated CRA cells was tested by Western blot analysis. Immunofluorescence (C) and flow cytometry (D) analysis of E-cadherin and vimentin expression in LovomiR-144-3p mimic cells with overexpression of ZEB1 or/and ZEB2, and in HCT116miR-144-3p inhibitor cells with knockdown of ZEB1 or/and ZEB2. (E) Representative IHC images of serial sections showed the expression of E-cadherin, vimentin, ZEB1, ZEB2 in NCMTs and CRATs with high miR-144-3p expression or low miR-144-3p expression determined by the cut-off value of qRT-PCR. *, P<0.05; **, P<0.01; ***, P < 0.001.