Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

Characterization of circular RNA (circRNA) expression profiles in adult and aged SkMSCs. (A) Volcano plots were used to evaluate differences in circRNA expression between adult and aged SkMSCs. The horizontal line represents a 2.0-fold (log2 scaled) difference in expression and the vertical lines represent P = 0.05 (–log10 scaled). (B) Hierarchical clustering of circRNA expression profiles. Red and green indicate high and low relative expression, respectively. The red and green points in the plot represent the significantly differentially expressed circRNAs. (C) circRNA FUT10 mRNA expression in the above two groups. (D) MTT assay showing adult and aged SkMSC proliferation. (E) Immunofluorescence analysis of MyHC and MyoD expression in adult and aged SkMSCs. (F) Quantification of MyHC and MyoD. Each bar represents the mean ± SEM. *P

Figure 1. Characterization of circular RNA (circRNA) expression profiles in adult and aged SkMSCs. (A) Volcano plots were used to evaluate differences in circRNA expression between adult and aged SkMSCs. The horizontal line represents a 2.0-fold (log2 scaled) difference in expression and the vertical lines represent P = 0.05 (–log10 scaled). (B) Hierarchical clustering of circRNA expression profiles. Red and green indicate high and low relative expression, respectively. The red and green points in the plot represent the significantly differentially expressed circRNAs. (C) circRNA FUT10 mRNA expression in the above two groups. (D) MTT assay showing adult and aged SkMSC proliferation. (E) Immunofluorescence analysis of MyHC and MyoD expression in adult and aged SkMSCs. (F) Quantification of MyHC and MyoD. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication within each individual experiment.