Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

Effect of circFUT10 on SkMSC proliferation and differentiation. (A) Adult SkMSCs were transfected with circRNA FUT10 overexpression vector, while aged SkMSCs were transfected with circFUT10 small interfering RNA for circRNA FUT10 knock down. circRNA FUT10 mRNA was analyzed using real-time qPCR. (B) SkMSC proliferation was measured by EdU assays. (C) Quantification of EdU-positive cells. (D) Western blot analysis showing MyHC and MyoD expression in different groups. (E) Quantification of MyHC and MyoD were normalized to β-actin. Each bar represents the mean ± SEM. *P

Figure 2. Effect of circFUT10 on SkMSC proliferation and differentiation. (A) Adult SkMSCs were transfected with circRNA FUT10 overexpression vector, while aged SkMSCs were transfected with circFUT10 small interfering RNA for circRNA FUT10 knock down. circRNA FUT10 mRNA was analyzed using real-time qPCR. (B) SkMSC proliferation was measured by EdU assays. (C) Quantification of EdU-positive cells. (D) Western blot analysis showing MyHC and MyoD expression in different groups. (E) Quantification of MyHC and MyoD were normalized to β-actin. Each bar represents the mean ± SEM. *P < 0.05, **P < 0.01. All experiments were performed at least three times with duplication within each individual experiment.