Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

miR-365a-3p regulates SkMSC proliferation and differentiation by targeting HOXA9. (A) SkMSCs were transfected with negative control (NC) miRNA, miR-365a-3P mimic, HOXA9-NC, HOXA9 overexpression vector (HOXA9-OE), and miR-365a-3p + HOXA9-OE. HOXA9 mRNA was examined by qPCR. (B) SkMSC proliferation was measured by EdU assays. (C) Quantification of EdU-positive cells. (D) MyHC and MyoD mRNA were examined by real-time qPCR. (E) Western blot analysis showing MyHC and MyoD protein expression in different groups. (F) Quantification of MyHC and MyoD were normalized to β-actin. *P

Figure 7. miR-365a-3p regulates SkMSC proliferation and differentiation by targeting HOXA9. (A) SkMSCs were transfected with negative control (NC) miRNA, miR-365a-3P mimic, HOXA9-NC, HOXA9 overexpression vector (HOXA9-OE), and miR-365a-3p + HOXA9-OE. HOXA9 mRNA was examined by qPCR. (B) SkMSC proliferation was measured by EdU assays. (C) Quantification of EdU-positive cells. (D) MyHC and MyoD mRNA were examined by real-time qPCR. (E) Western blot analysis showing MyHC and MyoD protein expression in different groups. (F) Quantification of MyHC and MyoD were normalized to β-actin. *P < 0.05, **P < 0.01. Each experiment was performed at least three times.