Research Paper Volume 13, Issue 13 pp 17428—17441

CircRNA FUT10 regulates the regenerative potential of aged skeletal muscle stem cells by targeting HOXA9

CircRNA FUT10 regulated HOXA9 by sponging miR-365a-3p. (A) SkMSCs were transfected with negative control (NC) miRNA, circRNA FUT10vector, miR-365a-3P mimic, (circ-OE), and circ-OE+miR-365a-3p. HOXA9 mRNA was examined by qPCR. (B) Western blot analysis showing HOXA9 protein expression in different groups. (C) Quantification of HOXA9 were normalized to β-actin. (D) SkMSC proliferation was measured by EdU assays. (E) Quantification of EdU-positive cells. (F) MyHC and MyoD mRNA were examined by real-time qPCR. (G) Western blot analysis showing MyHC and MyoD protein expression in different groups. (H) Quantification of MyHC and MyoD were normalized to β-actin. *P

Figure 8. CircRNA FUT10 regulated HOXA9 by sponging miR-365a-3p. (A) SkMSCs were transfected with negative control (NC) miRNA, circRNA FUT10vector, miR-365a-3P mimic, (circ-OE), and circ-OE+miR-365a-3p. HOXA9 mRNA was examined by qPCR. (B) Western blot analysis showing HOXA9 protein expression in different groups. (C) Quantification of HOXA9 were normalized to β-actin. (D) SkMSC proliferation was measured by EdU assays. (E) Quantification of EdU-positive cells. (F) MyHC and MyoD mRNA were examined by real-time qPCR. (G) Western blot analysis showing MyHC and MyoD protein expression in different groups. (H) Quantification of MyHC and MyoD were normalized to β-actin. *P < 0.05, **P < 0.01. Each experiment was performed at least three times.