Research Paper Volume 13, Issue 13 pp 17462—17472

miR-30a-5p suppresses lung squamous cell carcinoma via ATG5 - mediated autophagy

miR-30a-5p suppresses ATG5-mediated autophagy in LUSC. (A) DEmiRNA volcano map of siATG5 and siSRC SW900 group based. (B) Venn diagram of predicted upstream down-regulated DEmiRNAs for ATG5. (C) Pearson correlation analysis of ATG5 and miR-30a-5p; (D) The miR-30a-5p expression was down regulated in tumor group (red) compared with normal samples (blue); (E) Box plots of miR-30a-5p expression in different clinical stages of LUSC; (F) Survival curves of miR-30a-5p expression for prognosis. Red indicates high expression group and blue indicates low expression group; (G). Expression of miR-30a-5p in HPAEpic cell line and LUSC cell lines. (H) Binding sites of miR-30a-5p and 3‘UTR of ATG5; (I) Dual-luciferase reporter gene assay was used to determine the targeted binding of miR-30a-5p and ATG5.

Figure 3. miR-30a-5p suppresses ATG5-mediated autophagy in LUSC. (A) DEmiRNA volcano map of siATG5 and siSRC SW900 group based. (B) Venn diagram of predicted upstream down-regulated DEmiRNAs for ATG5. (C) Pearson correlation analysis of ATG5 and miR-30a-5p; (D) The miR-30a-5p expression was down regulated in tumor group (red) compared with normal samples (blue); (E) Box plots of miR-30a-5p expression in different clinical stages of LUSC; (F) Survival curves of miR-30a-5p expression for prognosis. Red indicates high expression group and blue indicates low expression group; (G). Expression of miR-30a-5p in HPAEpic cell line and LUSC cell lines. (H) Binding sites of miR-30a-5p and 3‘UTR of ATG5; (I) Dual-luciferase reporter gene assay was used to determine the targeted binding of miR-30a-5p and ATG5.