Research Paper Volume 13, Issue 13 pp 17462—17472

miR-30a-5p suppresses lung squamous cell carcinoma via ATG5 - mediated autophagy

Attenuation of miR-30a-5p/ATG5-regulated autophagy inhibits LUSC progression in lung metastatic model. (A) Mice were administered WT/siATG5/mimic miR-30a-5p/siATG5+mimic miR-30a-5p transfected SW900 cells (5 × 105/mouse) by i.p injection. Image representative gross appearance of lungs and livers from control (n = 3), siATG5 (n = 3), mimic miR-30a-5p (n = 3) and siATG5+mimic miR-30a-5p (n = 3) mice. (B) HE staining images of pulmonary metastatic samples in different group. (C) The body weight of mice in different groups. (D) Representative images of tumors from lung using immunofluorescence staining against DAPI P62, and LC3B. All the P values were compared with the control. *P **P

Figure 5. Attenuation of miR-30a-5p/ATG5-regulated autophagy inhibits LUSC progression in lung metastatic model. (A) Mice were administered WT/siATG5/mimic miR-30a-5p/siATG5+mimic miR-30a-5p transfected SW900 cells (5 × 105/mouse) by i.p injection. Image representative gross appearance of lungs and livers from control (n = 3), siATG5 (n = 3), mimic miR-30a-5p (n = 3) and siATG5+mimic miR-30a-5p (n = 3) mice. (B) HE staining images of pulmonary metastatic samples in different group. (C) The body weight of mice in different groups. (D) Representative images of tumors from lung using immunofluorescence staining against DAPI P62, and LC3B. All the P values were compared with the control. *P < 0.05, **P < 0.01.