Figure 3. ALK4 haplodeficency reduces pro-inflammation response. (A, B) ALK4 protein and iNOS expression in BMDM from WT and ALK4+/- mice in 4 groups (n=4 for each). (C) The mRNA expression of ALK4, iNOS, CCL2, IL-1β and COX-2 were detected in 4 groups (n=4 for each). (D, E) iNOS expression in LPS-stimulation BMDM from WT and ALK4+/- mice in immunofluorescence. * p<0.05. ** p<0.01. NS: not significant.