Research Paper Volume 13, Issue 12 pp 16834—16858

The consequences of a high-calorie diet background before calorie restriction on skeletal muscles in a mouse model

Generation of the experimental animals. CD-1 mice were fed ad libitum standard food (First stage) until they reached 12 months of age. Animals were separated into a group fed ad libitum standard food (SD) and ad libitum HCD (HC), for 17 weeks (Second stage). SD and HC animals were subdivided into control and experimental groups (Third stage). The experimental groups (SD-CR and HC-CR) were subjected to calorie restriction (CR) for 13 weeks before sacrifice.

Figure 1. Generation of the experimental animals. CD-1 mice were fed ad libitum standard food (First stage) until they reached 12 months of age. Animals were separated into a group fed ad libitum standard food (SD) and ad libitum HCD (HC), for 17 weeks (Second stage). SD and HC animals were subdivided into control and experimental groups (Third stage). The experimental groups (SD-CR and HC-CR) were subjected to calorie restriction (CR) for 13 weeks before sacrifice.