Figure 2. TSA inhibits adipogenesis of 3T3-L1 cells. Cells were incubated with a differentiation cocktail (DMI) medium with TSA (0.5 and 1 μM). (A) Cells were stained with Oil Red O on day 8; (B) Lipid accumulation was examined by measuring absorbance at 540 nm of Oil Red O staining. Scale bar, 100 μm (n=5-6, ####, P<0.0001 vs. vehicle group; &&, &&&, P<0.01, 0.001 vs. DMI treatment group).