Figure 6. TSA activates the AMPK pathway. (A) Cells were incubated with a differentiation cocktail (DMI) medium with TSA (1 μM). Phosphorylated and total levels of AMPKα were measured; (B) Cells were incubated with differentiation cocktail (DMI) medium with TSA (1 μM) or Compound C (10 μM) for 8 days. mRNA of PPAR-γ and C/EBPα; (C) Lipid accumulation was examined by measuring absorbance at 540 nm of Oil Red O (n=5-6, ####, P<0.0001 vs. vehicle group; &&&, P< 0.001 vs. DMI treatment group; $$$, P<0.001 vs. DMI+TSA group).