Research Paper Volume 13, Issue 13 pp 17499—17515

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis

Evaluation of the relationship between LINC00665 and miR-181c-5p, and miR-181c-5p and ZIC2. (A) The expression of miR-181c-5p was detected by RT-PCR after SK-LU-1 cells were transfected with mimic-miR-181c-5p or mimic-NC. (B) Predicted binding sites between LINC00665 and miR-181c-5p. (C) Luciferase gene reporter assay was carried out to verify whether miR-181c-5p was a target gene of LINC00665 in SK-LU-1 cells. (D) A schematic drawing indicated the putative binding sites between miR-181c-5p and ZIC2. (E) Luciferase gene reporter assay was carried out to verify whether ZIC2 was a target gene of miR-181c-5p in SK-LU-1 cells. (*p0.05).

Figure 3. Evaluation of the relationship between LINC00665 and miR-181c-5p, and miR-181c-5p and ZIC2. (A) The expression of miR-181c-5p was detected by RT-PCR after SK-LU-1 cells were transfected with mimic-miR-181c-5p or mimic-NC. (B) Predicted binding sites between LINC00665 and miR-181c-5p. (C) Luciferase gene reporter assay was carried out to verify whether miR-181c-5p was a target gene of LINC00665 in SK-LU-1 cells. (D) A schematic drawing indicated the putative binding sites between miR-181c-5p and ZIC2. (E) Luciferase gene reporter assay was carried out to verify whether ZIC2 was a target gene of miR-181c-5p in SK-LU-1 cells. (*p<0.05).