Research Paper Volume 13, Issue 13 pp 17499—17515

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis


Figure 5. Assessment of the function of LINC00665/miR-181c-5p axis in modulating LUAD cell growth. (A) The expression of LINC00665 was detected by RT-PCR after sh-LINC00665 was stably transfected into SK-LU-1 and Calu-3 cells. Stably transfected SK-LU-1 and Calu-3 cells (sh-NC + inhibitor-NC, sh-LINC00665 + inhibitor-NC, sh-LINC00665 + inhibitor-miR-181c-5p, sh-NC + inhibitor-miR-181c-5p) were collected and subjected to the following assays. (B, C) Cell viability was assessed by CCK-8 assay. (D, E) Cell clone formation was detected by clone formation assay. (*p<0.05, vs. sh-NC + inhibitor-NC group; #p<0.05, vs. sh-LINC00665 + inhibitor-NC group).