Research Paper Volume 13, Issue 13 pp 17499—17515

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis

Assessment of the function of LINC00665/miR-181c-5p axis in regulating LUAD cell invasion and ZIC2 expression. (A, B) Transwell chambers were applied for cell invasion assessment in sh-NC + inhibitor-NC, sh-LINC00665 + inhibitor-NC, sh-LINC00665 + inhibitor-miR-181c-5p, sh-NC + inhibitor-miR-181c-5p groups. (C, E) The mRNA level of ZIC2 was detected by RT-PCR technology. (D, F) The protein level of ZIC2 was measured by western blotting technology. (*p0.05, vs. sh-NC + inhibitor-NC group; #p0.05, vs. sh-LINC00665 + inhibitor-NC group).

Figure 6. Assessment of the function of LINC00665/miR-181c-5p axis in regulating LUAD cell invasion and ZIC2 expression. (A, B) Transwell chambers were applied for cell invasion assessment in sh-NC + inhibitor-NC, sh-LINC00665 + inhibitor-NC, sh-LINC00665 + inhibitor-miR-181c-5p, sh-NC + inhibitor-miR-181c-5p groups. (C, E) The mRNA level of ZIC2 was detected by RT-PCR technology. (D, F) The protein level of ZIC2 was measured by western blotting technology. (*p<0.05, vs. sh-NC + inhibitor-NC group; #p<0.05, vs. sh-LINC00665 + inhibitor-NC group).