Research Paper Volume 13, Issue 13 pp 17499—17515

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis

Knockdown of miR-181c-5p inhibited LUAD cell invasion by targeting ZIC2. (A, B) Transwell chambers were applied for cell invasion assessment. (C, E) The mRNA levels of ZIC2, HOXA1 and HOXA11 were detected by RT-PCR technology. (D, F) The protein levels of ZIC2, HOXA1 and HOXA11 were measured by western blotting technology. (*p0.05, vs. sh-NC + inhibitor-NC group; #p0.05, vs. sh-NC + inhibitor-miR-181c-5p group).

Figure 8. Knockdown of miR-181c-5p inhibited LUAD cell invasion by targeting ZIC2. (A, B) Transwell chambers were applied for cell invasion assessment. (C, E) The mRNA levels of ZIC2, HOXA1 and HOXA11 were detected by RT-PCR technology. (D, F) The protein levels of ZIC2, HOXA1 and HOXA11 were measured by western blotting technology. (*p<0.05, vs. sh-NC + inhibitor-NC group; #p<0.05, vs. sh-NC + inhibitor-miR-181c-5p group).