Figure 8. Knockdown of miR-181c-5p inhibited LUAD cell invasion by targeting ZIC2. (A, B) Transwell chambers were applied for cell invasion assessment. (C, E) The mRNA levels of ZIC2, HOXA1 and HOXA11 were detected by RT-PCR technology. (D, F) The protein levels of ZIC2, HOXA1 and HOXA11 were measured by western blotting technology. (*p<0.05, vs. sh-NC + inhibitor-NC group; #p<0.05, vs. sh-NC + inhibitor-miR-181c-5p group).