Research Paper Advance Articles

Glioma stem cell-derived exosomal miR-944 reduces glioma growth and angiogenesis by inhibiting AKT/ERK signaling

Overexpression of miR-944 inhibits angiogenesis of HUVECs. (A, B) Tube formation assay results show the number of branch points as an index of angiogenesis in blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. (C–G) Western blot analysis shows the relative expression levels of (C, D) VEGF, (C, E) angiogenin-1, (C, F) MMP9, and (C, G) MMP14 proteins in blank control, and agomiR-NC, agomiR-944, and antagomiR-944-transfected HUVECs. *P

Figure 3. Overexpression of miR-944 inhibits angiogenesis of HUVECs. (A, B) Tube formation assay results show the number of branch points as an index of angiogenesis in blank control, and agomiR-NC-, agomiR-944-, and antagomiR-944-transfected HUVECs. (CG) Western blot analysis shows the relative expression levels of (C, D) VEGF, (C, E) angiogenin-1, (C, F) MMP9, and (C, G) MMP14 proteins in blank control, and agomiR-NC, agomiR-944, and antagomiR-944-transfected HUVECs. *P < 0.05, **P < 0.01 vs. NC group. NC, negative control; HUVECs, human umbilical vein endothelial cells.