Figure 4. PAS staining to show changes in the tubular structures. PAS staining show differences between Non-transgenic rats (NTG), transgenic (TG), NTG-streptozotocin induced diabetes model (NTGSTZ), TG streptozotocin induced diabetes model (TGSTZ). STZ challenge triggered vacuolization (arrow) and degeneration of renal tubular epithelium NTGSTZ. TG rats that generally showed tubular dilation (arrow head) also showed higher levels of STZ induced vacuolization and degeneration. Scale bar represent 100 μm at 40 x magnification.