Research Paper Volume 13, Issue 13 pp 16957—16973

The dual distinct role of telomerase in repression of senescence and myofibroblast differentiation

TERT repressed αSMA expression in a p16 independent manner. (A) Control or p16 siRNA transfected cells were analyzed for the indicated proteins by western blotting. (B) Cells were transfected with pCMV-Myc-DDK-CDKN2A plasmid or control vector and analyzed as in (A). DDK (FLAG®) antibody was used to confirm the level of transfection. (C) The cells were transfected with p16 siRNA, followed by with H2O2 treatment for 48 hours, and then analyzed as in (A). The cell lysates were analyzed for αSMA and p16 protein by western blotting. GAPDH were used as the internal controls for all blots. The quantified protein expression level was expressed as relative integration units (RIU). Representative blots from 3 separate experiments are shown. *P **P

Figure 3. TERT repressed αSMA expression in a p16 independent manner. (A) Control or p16 siRNA transfected cells were analyzed for the indicated proteins by western blotting. (B) Cells were transfected with pCMV-Myc-DDK-CDKN2A plasmid or control vector and analyzed as in (A). DDK (FLAG®) antibody was used to confirm the level of transfection. (C) The cells were transfected with p16 siRNA, followed by with H2O2 treatment for 48 hours, and then analyzed as in (A). The cell lysates were analyzed for αSMA and p16 protein by western blotting. GAPDH were used as the internal controls for all blots. The quantified protein expression level was expressed as relative integration units (RIU). Representative blots from 3 separate experiments are shown. *P < 0.05. **P < 0.01.